Bisobanura iki mugihe Cryovial “Ntabwo ikoreshwa mugice cyamazi ya azote”?

Iyi nteruro ibaza ikibazo: “Noneho, ubu ni ubuhe bwoko bwa kirogenike iyo budashobora gukoreshwa muri azote yuzuye?”
Ntabwo icyumweru gishira ntidusabwa gusobanura ibi bisa nkibidasanzwe bigaragara kuri page yose yibicuruzwa bititaye kuwabikoze, tutitaye ku bunini kandi tutitaye niba umurongo wimbere wimbere cyangwa urudodo rwo hanze.
Igisubizo ni: Iki nikibazo cyinshingano ntabwo arikibazo kijyanye nubwiza bwa cryovial.
Reka dusobanure.
Kimwe na laboratoire iramba cyane, cryovial ikozwe mubushyuhe bwa polypropilene.
Ubunini bwa polypropilene bugena urwego rwubushyuhe butekanye.
Imiyoboro myinshi ya 15mL na 50mL ifite urukuta ruto rugabanya imikoreshereze yubushyuhe buri munsi ya -86 kugeza kuri 90 selisiyusi.
Urukuta ruto kandi rusobanura impamvu imiyoboro ya conm ya 15mL na 50mL itagirwa inama yo kuzunguruka ku gipimo cyihuta kirenga 15.000xg kuko plastiki ikunda gucikamo ibice kandi igacika iyo ikorewe kurenga iyi mbago.
Ibikoresho bya Cryogenic bikozwe muri polypropilene yuzuye ibemerera kwihagararaho munsi yubukonje bwinshi kandi bikazunguruka muri centrifuge kumuvuduko urenga 25.000xg cyangwa irenga.
Ikibazo kiri hamwe na kashe ya kashe ikoreshwa mukurinda cryovial.
Kugirango cryovial irinde neza ingirangingo, selile cyangwa virusi irimo, ingofero igomba kumanuka burundu hanyuma igakora kashe idasohoka.
Ikinyuranyo gito kizemerera guhumeka no kwanduza ingaruka.
Imbaraga zibabaza zikorwa nabakora cryovial kugirango bakore kashe nziza-nziza ishobora kuba irimo silikoni o-impeta na / cyangwa umugozi wimbitse kugirango umanure umupira neza.
Ngiyo urugero rwibyo uruganda rukora rushobora gutanga.
Ubwanyuma intsinzi cyangwa kunanirwa kwa cryovial kugirango ibungabunge icyitegererezo kugwa kuri tekinike ya laboratoire kugirango hamenyekane kashe nziza.
Niba kashe ikennye, ndetse no mugihe ingofero yafunzwe neza, azote yuzuye irashobora kwinjira muri cryovial mugihe yinjijwe mumazi ya azote.
Niba icyitegererezo gikonjeshejwe vuba, azote yuzuye irashobora kwaguka byihuse kandi igatera ibintu byotswa igitutu guturika no kohereza ibice bya pulasitike mumaboko no mumaso yabantu bose bibabaje kuba hafi.
Kubwibyo, usibye kubidasanzwe, abakora cryovial basaba ababagurisha kwerekana bashize amanga kwerekana ko badakoresheje cryovial zabo usibye icyiciro cya gaze ya azote yuzuye (hafi -180 kugeza -186C).
Urashobora guhita wihutisha ibintu bikonjesha muri cryovial ukoresheje igice cya azote yuzuye;biraramba bihagije kandi ntibizacika.
Ushaka kumenya byinshi ku ngaruka zo kubika amavatiri ya kirogenike muri azote yuzuye ya azote?
Dore ingingo yo mu kigo cya UCLA gishinzwe umutekano wa Laboratoire yerekana imvune kubera cryovial iturika.